ExM-STORM: Expansion Single Molecule Nanoscopy

Recently, Chen et al. invented Expansion Microscopy, or ExM, which imprints a specimen’s features of interest onto a polymer hydrogel network and expands the network homogeneously to magnify sub-diffraction-limit features. However, ExM bleaches massively, 50-100% of the fluorescent molecules; thus it is not suited for single-molecule imaging. Here, we describe improved ExM that is free of bleaching, and thus can be synergically integrated with single-molecule nanoscopy, STORM. . CC-BY 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not . http://dx.doi.org/10.1101/049403 doi: bioRxiv preprint first posted online Apr. 20, 2016;